ISI 28-1e Determination of Reducing Sugar, DE by Luff-Schoorl's method.
Return 1. Scope The method is applicable to all starch hydrolysis products. LT 1/9 1970 Rev.: LT 22.01.2002 2. Principle Iodine titration of excess copper 3. Reagents 3.1 Luff-Solution: 3.1.a Citric acid: 50 g C6C8O7,H2O p.a. is dissolved in 50 ml distilled water. 3.1.b. Sodium carbonate: 143.7 g Na2CO3 p.a. is dissolved in 400-500 ml boiling distilled water. 3.1.a + 3.1.b is gently mixed after cooling of 3.1.b 3.1.c Copper sulfate: 25 g CuSO4, 5 H2O p.a. is dissolved in approx. 100 ml distilled water and transferred to the mixture of (3.1.a + 3.1.b). Dilute to 1 liter w