Methods

Return 1. Scope The method is applicable to starch slurry   LT 15 Jan 1998 2. Principle Volume of starch sediment after centrifugation is interpreted as concentration of slurry   3. Apparatus 3.1 Centrifuge with free swinging rotor, manual or electrically powered 3.2 Centrifuge tube 15 ml with 0.1 ml marks 0-10 ml and mark at 15 ml   4. Procedure Fill tube (3.2) with 15.0 ml starch slurry. Spin in centrifuge until all starch has settled. remember counter weight

Return 1. Scope The method is applicable to starch   LT 1970 Rev. LT 7 Feb1999 2. Principle Starch size distribution is determined by screening   3. Apparatus 3.1 Screening set of lit, one or more screens and bottom 3.2 Laboratory scale 4. Procedure Weigh TARA of each screen and bottom Weigh 100 g starch sample and transfer to top screen. Shake stack of screens until fines through top screen is negligible. Remove top screen and shake unt

Return 1. Scope The method is applicable to starch in native or modified form.   KJSV Sep 1964 Rev.: LT 14 Jan 1998 2. Principle The sample is serial diluted, incubated and counted on agar plates   3. Apparatus 3.1 Electrical heated incubator thermostatically controlled 3.2 Petri dishes, approx. 9 cm in diameter use sterile disposable 3.3 Pipette 1000 microlitre with disposable tips or glass pipette 3.4 Pipette 100 microlitre with disposable tips or glass pipette 3.5 Laboratory balance

ISI 30-2e Determination of Heat Resistant Spore Count 1. Scope The method is applicable to granular starch in native or modified form.   LT 18 Dec 1964 Rev.: LT 30 Aug 1999 2. Principle The sample is serial diluted, incubated on peptone substrate, pasteurised and incubated   3. Apparatus 3.1 Electrical heated incubator thermostatically controlled 3.2 Pipette 2000 -3000 microlitre with disposable tips or glass pipette 3.3 Pipette 500 microlitre with disposable tips or glass pipette 3.4 Laboratory balance 3.5 Flask with wide opening, 100 ml. 3.5 Substrate tubes 160 mm x 16 mm with cap use sterile disposable 3.7 Autoclave Sterilise at 120 +/- 2  o C 20 min. 3.8 Bunsen burner   Work antiseptically throughout 4. Media 4.1 Peptone milk. Preparation of peptone milk 10 g peptone is dissolved in ½ litre fresh skimmed milk. Use heating as necessary. Cool and add up to 1 litre with skimmed milk. Add 10 ml 1% solution of bromcresolpurpur in alcohol. Dissolve 0.8 g cysteinhydrochloride in a small q

Return 1. Scope The method is applicable to all starch hydrolysis products.   LT 1/9 1970 Rev.: LT 22.01.2002 2. Principle Iodine titration of excess copper   3. Reagents 3.1 Luff-Solution: 3.1.a Citric acid: 50 g C6C8O7,H2O p.a. is dissolved in 50 ml distilled water. 3.1.b. Sodium carbonate: 143.7 g Na2CO3 p.a. is dissolved in 400-500 ml boiling distilled water.  3.1.a + 3.1.b is gently mixed after cooling of 3.1.b 3.1.c Copper sulfate: 25 g CuSO4, 5 H2O p.a. is dissolved in approx. 100 ml distilled water and transferred to the mixture of (3.1.a + 3.1.b). Dilute to 1 liter w